Xin Li,Hao Xu,Jianfeng Yi,Chunlu Dong,Hui Zhang,Zhengfeng Wang,Long Miao,Wence Zhou

J Cell Mol Med

 

AbstractClinical and experimental evidence indicates that tumour-associated macrophages support cancer progression. Moreover, macrophage-derived extracellular vesicles (EVs) are involved in pathogenesis of multiple cancers, yet the functions of molecular determinants in which have not been fully understood. Herein, we aim to understand whether macrophage modulates pancreatic ductal adenocarcinoma (PDAC) progres-sion in an EV-dependent manner and the underlying mechanisms. microRNA (miR)- 365 was experimentally determined to be enriched in the EVs from M2 macrophages (M2-EVs), which could be transferred into PDAC cells. Using a co-culture system, M2-EVs could enhance the proliferating, migrating and invading potentials of PDAC cells, while inhibition of miR-365 in M2-EVs could repress these malignant functions. B-cell translocation gene 2 (BTG2) was identified to be a direct target of miR-365, while the focal adhesion kinase (F/ATP)-dependent tyrosine kinase (AKT) pathway was activated by miR-365. We further demonstrated that overexpression of BTG2 could delay the progression of PDAC in vitro, whereas by impairing BTG2- mediated anti- tumour effect, M2-EV- miR- 365 promoted PDAC progression. For validation, a nude mouse model of tumorigenesis was established, in which we found that target-ing M2-EV- miR-365 contributed to suppression of tumour growth. Collectively, M2- EVs carry miR-365 to suppress BTG2 expression, which activated FAK/AKT pathway, thus promoting PDAC development.

URL:https://onlinelibrary.wiley.com/doi/10.1111/jcmm.16405